A medium was found in which manganese efficiently induces erythromycin-resistant mitochondrial mutations, and which is suitable for measuring Mn2+ uptake and the labelling of DNA (fig. 1). Mn2+ uptake is stimulated by glucose and slowed down by cycloheximide (Fig 2). Mg2+ competes with Mn2+ uptake m
Mutagenesis Assays in Yeast
โ Scribed by Gray F. Crouse
- Book ID
- 115639204
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 48 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1046-2023
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The induction of antibiotic-resistant mutations in yeast mitochondrial DNA by manganese is decreased when the manganese-containing medium is additionally supplemented with magnesium. At equimolar concentrations of manganese and magnesium the former is no longer mutagenic. Amino acid starvation, cycl
Evidence from the phenotype of mutants which partially block mutagenesis and from experiments made to time induced mutagenesis relative to cell division in yeast is used to question whether mutagenesis in yeast should be regarded as an error-prone repair phenomenon.