Manganese mutagenesis in yeast

✍ Scribed by Putrament, Aleksandra ;Baranowska, Hanna ;Ejchart, Anna ;Jachymczyk, Witold

• Book ID: 104777966
• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 596 KB
• Volume: 151
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00446914

✦ Synopsis

A medium was found in which manganese efficiently induces erythromycin-resistant mitochondrial mutations, and which is suitable for measuring Mn2+ uptake and the labelling of DNA (fig. 1). Mn2+ uptake is stimulated by glucose and slowed down by cycloheximide (Fig 2). Mg2+ competes with Mn2+ uptake much stronger than does Zn2+ (Fig. 3). All of the conditions which favour Mn2+ uptake also favour induction of erythromycin-resistant mutations (Tables 3, 4). Mn2+ strongly inhibits protein synthesis (Table 1). Nuclear DNA replication is also strongly inhibited by this cation, while mitochondrial DNA replication is only weakly inhibited during the first 3 h of labelling, but there is small if any increase of the label incorporation between the 3rd 6th h of labelling (Table 2). The relation between label incorporation into mitDNA and mutation induction by manganese is not straightforward (Table 5). From among 11 divalent cations tested, only Mn2+ was capable of inducing mitochondrial erythromycin-resistant mutations (Table 6).