Murine submandibular mucin (MSM): a mucin carrying N- and O-glycosylically bound carbohydrate-chains

✍ Scribed by Arie V. Nieuw Amerongen; Clasina H. Oderkerk; Pieter A. Roukema; Johannes H. Wolf; Jan J.W. Lisman; Bernard Overdijk

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 255 KB
• Volume: 115
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(83)88162-2

✦ Synopsis

The submandibular and sublingual salivary-glands of the mouse secrete mucins from their acinar cells'. Both mucins contained -25% of sialic acid and >l% of fucose and sulphate'. The submandibular mucin also contains substantial quantities of mannose'.

Moschera and Pigman obtained similar results for the rat sublingual mucm and it was suggested that both 0-and N-glycosylically bound oligosaccharide-chains might be present. We now report the isolation and composition of N-and 0-glycosylically bound carbohydratechains from murine submandibular mucin (MSM).

MSM was isolated essentially as described previously', and its purity was established by electrophoresis4 on 7.5% acrylamide gels at pH 8.9. The samples were solubilised either *To whom correspondence should be addressed.