𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Murine model of prosthesis failure for the long-term study of aseptic loosening

✍ Scribed by Shang-You Yang; Haiying Yu; Weiming Gong; Bin Wu; Lois Mayton; Richard Costello; Paul H. Wooley

Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
443 KB
Volume
25
Category
Article
ISSN
0736-0266

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We examined a novel mouse model of wear debris‐induced prosthesis instability and osteolysis, and its application for the evaluation of therapy. A stainless steel or titanium‐alloy pin was implanted into the proximal tibia to form a contiguous surface with the articular cartilage. In some mice, titanium particles were injected into the tibial canal during the surgery, followed by monthly intraarticular injection. MicroCT scans revealed that the implants without particle challenge were stable without bone mineral density changes for 6 months. Histological analysis showed new bone formation around the implant at 6 weeks postsurgery. Periprosthetic soft tissue with inflammatory cells was a ubiquitous finding at the interface between the implant and surrounding bone in samples exposed to titanium particles, and expression of IL‐1β, TNFα, and CD68 was common in these joints. Pullout tests indicated that an average 5N load was required to pull out stable implants from surrounding bone. However, particle stimulation dramatically reduced the pullout force to less than 0.4 N. The feasibility of in vivo gene transfer on this model was confirmed by X‐gal staining of synovial membrane and periprosthetic tissue after injection of AAV‐LacZ in the prosthetic joint. This murine model of weight‐bearing knee prosthesis provides an economical, reproducible, and easily obtained means to study joint arthroplasty failure. The ability to evaluate the biomechanical properties of the prosthetic joint, in addition to histological and biochemical examination, results in a useful model to investigate many of the properties of prosthetic joint components during the response to debris‐associated osteolysis. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:603–611, 2007

📜 SIMILAR VOLUMES

Experimental model for the long-term eff
Experimental model for the long-term effects of laser resurfacing
✍ Srdan Babovic; Melanie Triffet Trevino; Paul M. Petty 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 141 KB

## Abstract ## Background and Objective Evaluation of the long‐term effects of the laser resurfacing on development of the skin cancer after chronic ultraviolet B light exposure calls for the development of the appropriate animal model. ## Study Design/Materials and Methods The 27 C3H nu/nu nude

Modelling the long-term cost-effectivene
Modelling the long-term cost-effectiveness of endovascular or open repair for abdominal aortic aneurysm
✍ D. M. Epstein; M. J. Sculpher; A. Manca; J. Michaels; S. G. Thompson; L. C. Brow 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB 👁 2 views

## Abstract ## Background Recent randomized trials have shown that endovascular abdominal aortic aneurysm repair (EVAR) has a 3 per cent aneurysm-related survival benefit in patients fit for open surgery, but it also has uncertain long-term outcomes and higher costs. This study assessed the cost-e

Determinants of long term survival after
Determinants of long term survival after surgery for cancer of the lung : A population-based study
✍ Christine Bouchardy; Gérald Fioretta; Marc De Perrot; Mirjana Obradovic; Anastas 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 100 KB 👁 3 views

## BACKGROUND. Even if some determinants of lung cancer (LC) prognosis have been established, their independent effect on long term survival remains to be seen. The objective of the current study was to identify the prognostic indicators of long term survival among LC patients treated by surgery.

Hippocampal slice cultures integrated wi
Hippocampal slice cultures integrated with multi-electrode arrays: a model for study of long-term drug effects on synaptic activity
✍ Tarun K. Ahuja; John G. Mielke; Tanya Comas; Balu Chakravarthy; Geoffrey A.R. Me 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 412 KB

## Abstract Technological limitations have restricted the ability to determine chronic drug effects on synaptic function from in vitro preparations. In earlier studies, the extracellular recording duration was limited to less than 10 h and only a distinct population of neurons was examined. To addr