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Multiwell in-gel protein digestion and microscale sample preparation for protein identification by mass spectrometry

✍ Scribed by Malcolm G. Pluskal; Alla Bogdanova; Mary Lopez; Sara Gutierrez; Aldo M. Pitt


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
833 KB
Volume
2
Category
Article
ISSN
1615-9853

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Identification and detailed characterization of complex mixtures of proteins separated by polyacrylamide gel electrophoresis (PAGE) require optimized and robust methods for interfacing electrophoretic techniques to mass spectrometry. Peptide mapping by matrix-assisted laser desorption/ionization-tim