A leaf disc method is described to permit the localized incorporation of (35)S-L-methionine into polypeptides synthesized in individual leaves of maize plants grown in the field. The method of incorporation employs minimal external manipulation of the intact leaf, is simple, repeatable, and may be u
Multivariate analyses of polypeptide synthesis in developing maize embryos
β Scribed by J. G. Boothe; D. B. Walden
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 744 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0040-5752
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β¦ Synopsis
Variation in polypeptide synthesis was examined in developing maize embryos of two inbred and two hybrid genotypes. Multivariate analyses were used to evaluate the variation among two-dimensional, electrophoretic separations of polypeptides. Several features of the data set were revealed. Similar developmental patterns were exhibited by all genotypes and no evidence was obtained for differential rates of development for inbreds and hybrids. The differential synthesis of two subsets of polypeptides during embryo development was observed. The multivariate methods employed in this study were a valuable aid in interpreting the results from a large and complex data set.
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