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Multiprotein Expression Strategy for Structural Biology of Eukaryotic Complexes

✍ Scribed by Daniel J. Fitzgerald; Christiane Schaffitzel; Philipp Berger; Ralf Wellinger; Christoph Bieniossek; Timothy J. Richmond; Imre Berger

Book ID
113918755
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
515 KB
Volume
15
Category
Article
ISSN
0969-2126

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The production of sufficient quantities of protein is an essential prelude to a structure determination, but for many viral and human proteins this cannot be achieved using prokaryotic expression systems. Groups in the Structural Proteomics In Europe (SPINE) consortium have developed and implemented