๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Ratiocinative screen of eukaryotic integral membrane protein expression and solubilization for structure determination

โœ Scribed by Franklin A. Hays; Zygy Roe-Zurz; Min Li; Libusha Kelly; Franz Gruswitz; Andrej Sali; Robert M. Stroud

Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
251 KB
Volume
10
Category
Article
ISSN
1345-711X

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

Rapid screening for structural integrity
Rapid screening for structural integrity of expressed proteins by heteronuclear NMR spectroscopy
โœ Angela M. Gronenborn; G. Marius Clore ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› Cold Spring Harbor Laboratory Press ๐ŸŒ English โš– 284 KB

A simple and rapid method based on 15N labeling and 1H-15N heteronuclear single quantum coherence spectroscopy is presented to directly assess the structural integrity of overexpressed proteins in crude Escherichia coli extracts without the need for any purification. The method is demonstrated using

Construction of a series of vectors for
Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins fromMycobacterium tuberculosis
โœ Huajun Qin; Jian Hu; Yuanzhi Hua; Shridhar V Challa; Timothy A Cross; Fei P Gao ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› BioMed Central ๐ŸŒ English โš– 899 KB

## Background One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clo