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Multiplex real-time PCR assay for simultaneous quantitation of human cytomegalovirus and herpesvirus-6 in polymorphonuclear and mononuclear cells of transplant recipients

✍ Scribed by Karine Pradeau; Ludovic Couty; Jean-Christophe Szelag; Pascal Turlure; Florence Rolle; Pierre Ferrat; Dominique Bordessoule; Yann Le Meur; François Denis; Sylvie Ranger-Rogez


Book ID
113767150
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
161 KB
Volume
132
Category
Article
ISSN
0166-0934

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## Abstract Human cytomegalovirus (HCMV) infections and anti‐HCMV treatment are usually monitored by measuring pp65 antigenemia. This method is time‐consuming, labour‐intensive and requires skilled operators. We have compared results obtained using real‐time Light Cycler™ quantitative PCR (QPCR) an