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Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

โœ Scribed by Sakuma, Tetsushi; Nishikawa, Ayami; Kume, Satoshi; Chayama, Kazuaki; Yamamoto, Takashi


Book ID
124161899
Publisher
Springer Science and Business Media LLC
Year
2014
Tongue
English
Weight
915 KB
Volume
4
Category
Article
ISSN
2045-2322

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โœ Ghorbal, Mehdi; Gorman, Molly; Macpherson, Cameron Ross; Martins, Rafael Miyazaw ๐Ÿ“‚ Article ๐Ÿ“… 2014 ๐Ÿ› Nature Publishing Group ๐ŸŒ English โš– 533 KB

Malaria causes ~660,000 deaths per year, and P. falciparum is the protozoan responsible for its most severe form. The lack of effective vaccines and the parasite's ability to develop drug resistance are major barriers to malaria treatment and eradication. Classical genome manipulation in P. falcipar