Multiple Myeloma: Methods and Protocols

✍ Scribed by Ross D. Brown, P. Joy Ho

• Publisher: Humana Press
• Year: 2005
• Tongue: English
• Leaves: 319
• Series: Methods in Molecular Medicine
• Edition: 1
• Category: Library

✦ Synopsis

A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicineв„ў series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.

✦ Table of Contents

Front......Page 1
Other Titles in Series......Page 2
Preface......Page 5
Contents......Page 7
Contributors......Page 9
1 Multiple Myeloma - Challenges and Opportunities......Page 13
2 Immunophenotyping of Plasma Cells in Multiple Myeloma......Page 17
3 Plasma Cell Labeling Index......Page 37
4 Conventional Cytogenetics in Myeloma......Page 49
5 Multicolor Spectral Karyotyping in Multiple Myeloma......Page 61
6 Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization......Page 71
7 Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma......Page 83
8 Southern Blotting of IgH Rearrangements in B-Cell Disorders......Page 97
9 Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma......Page 117
10 Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma......Page 133
11 Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma......Page 157
12 Incomplete DJH Rearrangements......Page 177
13 Identification of Malignant Plasma Cells by mRNA In Situ Hybridization......Page 187
14 The SCID-hu Myeloma Model......Page 195
15 The 5T2MM Murine Model of Multiple Myeloma......Page 203
16 Determination of Telomerase Activity and Telomere Length......Page 219
17 Dendritic Cell/Myeloma Hybrid Vaccine......Page 237
18 Genetically Engineered Myeloma Cell Vaccine......Page 247
19 An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors......Page 257
20 Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma......Page 269
21 Detecting Mismatch Repair Defects in Myeloma......Page 281
22 Methylation-Specific Polymerase Chain Reaction......Page 291
23 Making Sense of DNA Microarray Data......Page 305
Index......Page 317
Back......Page 319