Multiple Myeloma

✍ Scribed by Christoph Heuck, Niels Weinhold

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 217
• Series: Methods in Molecular Biology 1792
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume provides a thorough guide of the study of multiple myeloma (MM)—from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in this book cover topics, such as next-generation-flow-based methods for detection of minimal residual disease and circulating tumor cells; Cytometry by Time-of-Flights (CyTOF); a droplet digital PCR approach for finding contamination in patients’ samples; genome-wide investigation of the methylation and hydroxymethylation status of cytosines; and a microfluidic approach for creating nanoparticles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Multiple Myeloma: Methods and Protocols is a valuable resource for molecular scientists and laboratory supervisors working with MM samples, and any investigator conducting studies in this field.

✦ Table of Contents

Front Matter ....Pages i-x
ELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral Blood (Stefanie Huhn)....Pages 1-14
EuroFlow-Based Next-Generation Flow Cytometry for Detection of Circulating Tumor Cells and Minimal Residual Disease in Multiple Myeloma (Leire Burgos, Bruno Paiva)....Pages 15-34
Cytoplasmic Immunoglobulin Vs. DNA Analysis by Flow Cytometry (Daisy Alapat)....Pages 35-45
Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF) (Tina Smets, Frederik Stevenaert, Homer Adams III, Greet Vanhoof)....Pages 47-54
Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma (Erming Tian)....Pages 55-69
Whole Exome Sequencing in Multiple Myeloma to Identify Somatic Single Nucleotide Variants and Key Translocations Involving Immunoglobulin Loci and MYC (Brian A. Walker)....Pages 71-95
RNA-Sequencing from Low-Input Material in Multiple Myeloma for Application in Clinical Routine (Anja Seckinger, Tomi Bähr-Ivacevic, Vladimir Benes, Dirk Hose)....Pages 97-115
Protocol for M3P: A Comprehensive and Clinical Oriented Targeted Sequencing Panel for Routine Molecular Analysis in Multiple Myeloma (Santiago Barrio, Matteo DáVia, Laura Bruins, Thorsten Stühmer, Torsten Steinbrunn, Max Bittrich et al.)....Pages 117-128
Analysis of Circulating Tumor DNA (Sridurga Mithraprabhu, Andrew Spencer)....Pages 129-145
Detection of Cross-Sample Contamination in Multiple Myeloma Samples and Sequencing Data (Owen W. Stephens, Tobias Meißner, Niels Weinhold)....Pages 147-155
Analysis of Global Gene Expression Profiles (Alboukadel Kassambara, Jerome Moreaux)....Pages 157-166
Genome Wide Mapping of Methylated and Hydroxyl-Methylated Cytosines Using a Modified HpaII Tiny Fragment Enrichment by Ligation Mediated PCR Tagged Sequencing Protocol (Sanchari Battachariyya, Ruslana Tytarenko, Christoph Heuck, John Greally, Amit Verma)....Pages 167-177
A Rapid and Robust Protocol for Reduced Representation Bisulfite Sequencing in Multiple Myeloma (Samrat Roy Choudhury, Brian A. Walker)....Pages 179-191
Microfluidic Production and Application of Lipid Nanoparticles for Nucleic Acid Transfection (Anitha Thomas, Shyam M. Garg, Rebecca A. G. De Souza, Eric Ouellet, Grace Tharmarajah, Dave Reichert et al.)....Pages 193-203
Microfluidic Assembly of Liposomes with Tunable Size and Coloading Capabilities (Jessica R. Hoffman, Ennio Tasciotti, Roberto Molinaro)....Pages 205-214
Back Matter ....Pages 215-216

✦ Subjects

Biomedicine; Cancer Research