Multiple location of K-ATPase in maize coleoptiles
β Scribed by Hendriks, Thijs
- Book ID
- 122733425
- Publisher
- Elsevier Science
- Year
- 1977
- Weight
- 645 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0304-4211
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The plasma-membrane H Β§ (EC 3.6.1.35) of maize (Zea mays L.) coleoptiles and enclosed leaves has been localized at the optical-microscope level utilizing paraffin sections and a specific monoclonal antibody. Both in coleoptiles and in leaves the stomatal guard cells and the phloem of vascular bundle
The calmodulin-stimulated ATPase of maize (Zea mays L.) coleoptiles has been purified by calcium-dependent binding to a calmodulin affinity column. In the presence of protease inhibitors (phenylmethylsulfonylfluoride and chymostatin) a polypeptide of relative molecular mass (Mr) 140000 (Β±10000) is o