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Immunocytolocalization of plasma-membrane H+-ATPase in maize coleoptiles and enclosed leaves

✍ Scribed by Jose M. Villalba; Martin Lützelschwab; Ramón Serrano

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
1020 KB
Volume
185
Category
Article
ISSN
0032-0935

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✦ Synopsis

The plasma-membrane H § (EC 3.6.1.35) of maize (Zea mays L.) coleoptiles and enclosed leaves has been localized at the optical-microscope level utilizing paraffin sections and a specific monoclonal antibody. Both in coleoptiles and in leaves the stomatal guard cells and the phloem of vascular bundles are the tissues most enriched in ATPase. The enrichment in guard cells is relevant to active ion transport during stomata opening. Considering the postulated activation of coleoptile ATPase by auxin, it is remarkable that the ATPase is not enriched in the coleoptile epidermis, where most of the auxin receptor is located.

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