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Multiple catalytic activities of Escherichia coli lysyl-tRNA synthetase (LysU) are dissected by site-directed mutagenesis

โœ Scribed by Chen, Xiaolong; Boonyalai, Nonlawat; Lau, Catherine; Thipayang, Salinthip; Xu, Yuhong; Wright, Michael; Miller, Andrew D.


Book ID
119828691
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
605 KB
Volume
280
Category
Article
ISSN
1432-1327

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โœ Fersht, Alan R. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› American Chemical Society ๐ŸŒ English โš– 828 KB

To understand an enzyme reaction, one has to characterize the bound substrates, intermediates, products, and transition states on the reaction pathway and determine the interaction energies between them and the enzyme as the reaction proceeds. Site-directed mutagenesis is invaluable in this task, en