Multiple and novel specificities of monoclonal antibodies O1, O4, and R-mAb used in the analysis of oligodendrocyte development

✍ Scribed by R. Bansal; A. E. Warrington; A. L. Gard; B. Ranscht; S. E. Pfeiffer

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 997 KB
• Volume: 24
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490240413

✦ Synopsis

Three monoclonal antibodies that react with antigens on the surface of developing oligodendrocytes in a stage-specific manner, 01, 0 4 (Sommer and Schachner, 1981), and R-mAb , have been studied with respect to their specificities for a number of purified lipids. The observed specificities were consistent regardless of how the antigens were presented to the antibodies. 01 reacted with galactocerebroside, monogalactosyl-diglyceride, and psychosine and, in addition, labeled an unidentified species in rat brain extracts. R-mAb reacted with galactocerebroside, monogalactosyl-diglyceride, sulfatide, seminolipid, and psychosine; the reaction of R-mAb with sulfatide was nearly equal to that with galactocerebroside. 0 4 reacted with sulfatide, seminolipid, and to some extent with cholesterol. However, oligodendrocyte progenitor cells labeling with 0 4 that had not yet begun to express the 01 antigen failed to incorporate 35S0, or [3H]galactose into sulfatide or seminolipid, the syntheses of which first appear in Ol-positive cells. Therefore, 0 4 stains, in addition to sulfatide and seminolipid, an unidentified antigen that appears on the surface of oligodendrocyte progenitors prior to the expression of sulfatide and galactocerebroside. In primary cultures of rat brain, developing 0 4 + oligodendrocyte progenitors stained slightly earlier with R-mAb than with 0 1 , and thus R-mAb transiently stained a larger population of oligodendrocytes than did 01. None of the three antibodies produced a detectable reaction on Western immunoblot after separation of brain proteins on reducing gels. In conclusion, the results show that 0 4 , R-mAb, and 0 1 have multiple overlapping specificities, including previously unrecognized cross-reactions.