Novel stage in the oligodendrocyte lineage defined by reactivity of progenitors with R-mAb prior to O1 anti-galactocerebroside
✍ Scribed by R. Bansal; S. E. Pfeiffer
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 727 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
The developmentally regulated appearance of surface immuno‐reactivity of proligodendroblasts [oligodendrocyte progenitors reacting with monoclonal antibodies A007 and O4, but not anti‐galactocerebroside (GalC), i.e., A007/O4^+^ GalC^−^] to monoclonal antibodies R‐mAb and O1 was studied both in culture and in vivo. In both cases staining with R‐mAb shortly preceded that with O1; that is, a transient population of R‐mAb^+^ O1^−^ cells was observed. R‐mAb^−^ O1^+^ cells were not detected. Differential staining with R‐mAb and O1 was also noted at the subcellular level. In younger cultures in which R‐mAb^+^ cells were first acquiring O1 immunoreactivity, many of these cells were stained by O1 only on the cell bodies and proximal portions of the processes, whereas in contrast R‐mAb stained the whole cell, including the distal portions of the processes. Only in older, more mature R‐mAb^+^ cells did O1 also stain the distal portions of processes. The expression of reactivity to R‐mAb and O1 was compared to the proliferative capacity of the cells. Proliferation [assessed by bromodeoxyuridine (BrdU) incorporation] of both R‐mAb^+^ and O1^+^ cells was negligible both in culture and in vivo. However, treatment of cells in culture with 10 ng/ml basic fibroblast growth factor resulted in an enhancement of proliferation of the R‐mAb^+^ cells. Within the proliferating R‐mAb^+^BrdU^+^ population, 80% of the cells were O1^−^ (i.e., anti‐galactocerebroside negative). These events occur during a critical period of development when A007/O4^+^ proligodendroblasts begin to become post‐mitotic and express surface galactocerebroside. The data demonstrate that the use of R‐mAb as an “anti‐GalC” must be interpreted with caution, and indicate the utility of dual staining with R‐mAb and O1 to (1) further subdivide the oligodendrocyte lineage, thus identifying an additional, GalC^−^ developmental compartment, and (2) observe the distribution of R‐mAb and O1 immunoreactivity at a subcellular level. © 1992 Wiley‐Liss, Inc.