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Multiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry

✍ Scribed by Doris Wiener; David T. Rowlands; Nancy Lowell; Shirish Shah; John Malone; Saul Lowitt

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 341 KB
Volume: 4
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860040305

No coin nor oath required. For personal study only.

✦ Synopsis

Lymphocyte subset analysis was performed on 114 healthy children and 84 healthy adults. Samples were prepared by a whole blood lysis technique and analyzed by flow cytometry. The percentage and total number of CD3 + , CD4 + , CD8 + , and CD19 + lymphocytes were calculated for each of six age groups. A direct correlation with age was seen in the percentages of CD3 + , CD4 + , and CD8 + lymphocytes. The absolute number and percentage of total lymphocytes, the percentage and absolute number of CD19+ lymphlocytes, and the absolute number of CD3 + lymphocytes decreased with age. No significant correlation with age was observed for white blood cells, the absolute number of C D 4 t and CD8+ lymphocytes, and the CD4 +/CD8 + ratio.

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