Mitochondrial membrane potential (MMP) in dissociated rat cerebellar neurons was measured using rhodamine 123 (Rh 123) as fluorescent dye, and flow cytometry. Dye distribution was studied by confocal scanning microscopy. Propidium iodide (PI)-marked cells (dead cells) were not stained by Rh 123, whi
✦ LIBER ✦
Analysis of mitochondrial membrane potential in the cells by microchip flow cytometry
✍ Scribed by Masatoshi Kataoka; Yoko Fukura; Yasuo Shinohara; Yoshinobu Baba
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 315 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
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Background: Modifications of intracellular transfer, resulting from a loss of membrane integrity may contribute toward setting the cell onto the pathway of apoptosis. Methods: We have developed an original technique of measuring simultaneously, with flow cytometry, changes in membrane fluidity and c