Analysis of the cell cycle by flow cytometry
β Scribed by Jozsef Szeberenyl
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2007
- Tongue
- English
- Weight
- 70 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1470-8175
- DOI
- 10.1002/bmb.41
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Flow cytometry was used to measure cell cycle parameters in Solanum aviculare plant cell suspensions. Methods for bromodeoxyuridine (BrdU) labeling of plant nuclei were developed so that cell cycle times and the proportion of cells participating in growth could be determined as a function of culture
The simultaneous detection of the green fluorescent protein (GFP) and DNA content using propidium iodide (PI) by flow cytometry is made difficult because of the unique nature of these 2 fluorogenic reagents. For PI to enter cells efficiently and to stain DNA quantitatively, the cells must first be p
Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while