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Multidimensional protein fractionation of blood proteins coupled to data-independent nanoLC–MS/MS analysis

✍ Scribed by Yishai Levin; Julian A.J. Jaros; Emanuel Schwarz; Sabine Bahn

Book ID: 104027514
Publisher: Elsevier
Year: 2010
Tongue: English
Weight: 343 KB
Volume: 73
Category: Article
ISSN: 1874-3919
DOI: 10.1016/j.jprot.2009.10.013

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## Abstract To improve the efficiency of proteome analysis, a strategy with the combination of protein pre‐fractionation by preparative microscale solution isoelectric focusing, peptide separation by μRPLC with serially coupled long microcolumn and protein identification by ESI‐MS/MS was proposed.