## Abstract Summary: A successful embryo transfer depends on the quality of the transferred embryos, recipients, and the transfer techniques. Among these, transfer techniques are often the limiting factor because transfer methodologies and personal skills vary. Suboptimal embryo transfer procedures
Mouse embryos develop normallyExo utero
โ Scribed by Muneoka, K. ;Wanek, N. ;Bryant, S. V.
- Book ID
- 102336401
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 384 KB
- Volume
- 239
- Category
- Article
- ISSN
- 0022-104X
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โฆ Synopsis
Surgical procedures are described which permit direct experimental access to postimplantation mouse embryos. These procedures arose from our finding that development proceeds normally to term after embryos have been released from the confines of the uterus, while remaining attached to it via the placenta. Embryos continue to develop exo utero, within the abdominal cavity of the mother, and are capable of surviving a variety of different surgical manipulations. This finding opens the way for experimental analyses of mouse development in vivo.
๐ SIMILAR VOLUMES
A high resolution ultrasound imaging technique, ultrasound backscatter microscopy (UBM), has previously been shown to be useful for in utero imaging of mouse embryos, and for direct manipulation of mouse embryos through UBM-guided injections. UBM images from mouse embryos staged between 8.5 and 10.5