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In utero ultrasound backscatter microscopy of early stage mouse embryos

โœ Scribed by Daniel H Turnbull

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
769 KB
Volume
23
Category
Article
ISSN
0895-6111

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โœฆ Synopsis

A high resolution ultrasound imaging technique, ultrasound backscatter microscopy (UBM), has previously been shown to be useful for in utero imaging of mouse embryos, and for direct manipulation of mouse embryos through UBM-guided injections. UBM images from mouse embryos staged between 8.5 and 10.5 days of gestation are presented to demonstrate the range of anatomical structures which can be studied with this approach. Ultrasound contrast agents have been injected into the forebrain ventricle of 10.5 day embryos to characterize the resulting three-dimensional distribution of the injected agents. These studies provide important background data relevant to future use of this technique for in utero analysis of early brain and heart development, and for in utero manipulation of mouse embryos through UBM-guided injections.

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