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Mounting Live Cells Attached to Coverslips for Microscopy

โœ Scribed by Fischer, Andrew H. (author);Jacobson, Kenneth A. (author);Rose, Jack (author);Zeller, Rolf (author)


Book ID
118183876
Publisher
Cold Spring Harbor Laboratory Press
Year
2008
Tongue
English
Weight
65 KB
Volume
2008
Category
Article
ISSN
1940-3402

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Background: For high-resolution microscopy, cells have to be analyzed through thin glass coverslips. Therefore, it is necessary to culture cells on coverslips for preservation of cell morphology. We found cell attachment and spreading to be relatively slow processes, even when cells were plated on c

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A variety of research applications require the use of cultured cells. However, it often is difficult to obtain well embedded monolayers of cultured cells for ultrastructural analysis, especially if sections parallel to the cell monolayer are desirable. Previously published techniques for obtaining p