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Microscopy: STED for living cells

✍ Scribed by Won, Rachel

Book ID
118009370
Publisher
Nature Publishing Group
Year
2011
Tongue
English
Weight
209 KB
Volume
5
Category
Article
ISSN
1749-4885

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## Abstract We compare the performance of video‐rate Stimulated Emission Depletion (STED) and confocal microscopy in imaging the interior of living neurons. A lateral resolution of 65 nm is observed in STED movies of 28 frames per second, which is 4‐fold higher in spatial resolution than in their c