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Nanoscale imaging of living synapses by STED microscopy

✍ Scribed by Valentin Nägerl

Book ID
116776489
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
71 KB
Volume
65
Category
Article
ISSN
0168-0102

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## Abstract We compare the performance of video‐rate Stimulated Emission Depletion (STED) and confocal microscopy in imaging the interior of living neurons. A lateral resolution of 65 nm is observed in STED movies of 28 frames per second, which is 4‐fold higher in spatial resolution than in their c