Morphological measurements on filamentous microorganisms by fully automatic image analysis

✍ Scribed by H. L. Packer; C. R. Thomas

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 822 KB
• Volume: 35
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260350904

✦ Synopsis

Characterization of mycelial morphology is important for physiological and engineering studies of filamentous fermentations, and in the design and operation of such fermentations. Image analysis has been developed as a method for this characterization, and has been shown to be faster and generally more accurate than previous methods. A fully automatic system has been developed, in which speed is gained, but with loss of accuracy in some cases. The method has been tested on Streptornyces clavuligerus and Penicillium chrysogenum P1 batch fermentations. It has also been tested on a fedbatch Penicillium chrysogenurn P2 fermentation, in which the medium contained solid ingredients. Fully automatic image analysis for morphological characterization of filamentous microorganisms is an important development which will make practical many engineering and physiological studies of such fermentations that have so far not been completely satisfactory.