## Abstract Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15–18 hr of sper
Morphological development and sex of bovine in vitro-fertilized embryos
✍ Scribed by Dr. Birthe Avery; Claus B. Jørgensen; Valerie Madison; Torben Greve
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 578 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1040-452X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Bovine in vitro‐fertilized embryos at the blastocyst stage were collected at days 7, 8, and 10 postinsemination and sex was determined via the polymerase chain reaction (PCR) to compare the embryonic development with the sex of the embryos. The percentages of males (sex ratio) after division of the embryos into three developmental groups were 68%, 48%, and 35% in the fast, intermediate, and slow groups, respectively (P = 0.014). The percentages of males on days 7, 8, and 10 were 60%, 40% and 33%, respectively (P = 0.043). The average sex ratio for the whole material was 50%. It is thus concluded that male bovine preimplantation embryos develop faster than female embryos.
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