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In vitro fertilization and preimplantation embryo development of African green monkeys (Cercopithecus aethiops)

✍ Scribed by T. Sankai; F. Cho; Y. Yoshikawa

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 109 KB
Volume: 43
Category: Article
ISSN: 0275-2565
DOI: 10.1002/(sici)1098-2345(1997)43:1<43::aid-ajp3>3.0.co;2-y

No coin nor oath required. For personal study only.

✦ Synopsis

Ovaries of five adult female African green monkeys were stimulated by repeated administrations of equine chorionic gonadotrophin (eCG), followed by a single administration of human chorionic gonadotrophin (hCG). Oocytes were collected from enlarged follicles 28 h after hCG administration and incubated in vitro for 288 h. Oocytes that had extruded the first polar body were inseminated with spermatozoa that had been incubated for 4 to 6 h in medium with caffeine and dibutyryl cyclic AMP. Of these oocytes, 66% were fertilized and the incidence of polyspermy was 37%. Eighty-two percent of the fertilized eggs cleaved, with some developing into expanded blastocysts.

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