Morphogenesis in Isolated Microspore Cultures ofBrassica juncea
β Scribed by Nidhi P. Chanana; Vibha Dhawan; Sant S. Bhojwani
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 410 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0167-6857
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Direct microspore-derived embryo formation in anther cultures of two cultivars of Brassica juncea was obtained. Preliminary culture of anthers at 35 Β°C for 1-5 days prior to maintenance at 25 Β°C stimulated embryogenesis. Embryogenesis was also stimulated by an initial culture at 5 Β°C for 3 days. Ana
An efficient culture system has been developed for repeated cycles of somatic embryogenesis in microspore-derived embryos of Brassica juncea without a callus phase. Haploid embryos produced through anther culture showed a high propensity for direct production of somatic embryos in response to 2 mgL(