Microspore embryogenesis in anther cultures of two Indian cultivars ofBrassica juncea(L.) Czern.

✍ Scribed by K. K. Sharma; Sant S. Bhojwani

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 223 KB
• Volume: 4
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00040197

✦ Synopsis

Direct microspore-derived embryo formation in anther cultures of two cultivars of Brassica juncea was obtained. Preliminary culture of anthers at 35 °C for 1-5 days prior to maintenance at 25 °C stimulated embryogenesis. Embryogenesis was also stimulated by an initial culture at 5 °C for 3 days. Analysis of squashed anthers revealed that approximately 10% of the microspores began dividing, but less than 1% developed into macroscopic embryos. All embryos transferred to embryo culture medium survived, but only 30% of these developed directly into normal plantlets. The androgenic plants were haploid (2n = 18).