✦ LIBER ✦

Monoparametric models of flow cytometric karyotypes with spreadsheet software

✍ Scribed by J. Conia; P. Muller; S. Brown; C. Bergounioux; P. Gadal

Book ID: 104692407
Publisher: Springer
Year: 1989
Tongue: English
Weight: 742 KB
Volume: 77
Category: Article
ISSN: 0040-5752
DOI: 10.1007/bf00266200

No coin nor oath required. For personal study only.

✦ Synopsis

Theoretical flow karyotypes from both plant and mammalian species have been simply modelled using computer spreadsheet software. The models are based upon published values of relative DNA content or relative lengths of each of the chromosomes. From such data, the histograms of chromosome distribution have been simulated for both linear and logarithmic modules of a flow cytometer, and as a function of the coefficient of variation. Simulated and experimental histograms are compard for Nicotiana plumbaginifolia. This readily accessible exercise facilitates the planning and execution of flow cytometric analysis and sorting of chromosomes.

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