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Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion

✍ Scribed by Djuro Josic; Andrea Buchacher; Alois Jungbauer

Book ID: 108447684
Publisher: Elsevier Science
Year: 2001
Weight: 772 KB
Volume: 752
Category: Article
ISSN: 0378-4347
DOI: 10.1016/s0378-4347(00)00499-0

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## Abstract A CEC weak cation‐exchange monolith has been prepared by __in situ__ polymerization of acrylamide, methylenebisacrylamide and 4‐acrylamidobutyric acid in a decanol–dimethylsulfoxide mixture as porogen. The columns were evaluated by SEM and characterized with regard to the separation of