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Monoclonal antibodies to human cartilage oligomeric matrix protein: epitope mapping and characterization of sandwich ELISA

✍ Scribed by Vladimı́r Vilı́m; Zdeněk Vobůrka; Richard Vytášek; Ladislav Šenolt; Ilja Tchetverikov; Virginia B Kraus; Karel Pavelka


Book ID
117074821
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
284 KB
Volume
328
Category
Article
ISSN
0009-8981

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Of nine mouse monoclonal antibodies (MAbs) directed against the lower matrix protein (pp65; ppUL83) of human cytomegalovirus (HCMV), all immunoprecipitated the 65-kDa protein. Only five were reactive by Western blotting, however, and four of these mapped to linear antigenic epitopes located between