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Monitoring of spatial expression of firefly luciferase in transformed zebrafish

✍ Scribed by Mayerhofer, R. ;Araki, K. ;Szalay, A. A.

Publisher
John Wiley and Sons
Year
1995
Weight
584 KB
Volume
10
Category
Article
ISSN
0884-3996

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✦ Synopsis

The firefly luciferase gene attached t o the cytomegalovirus promoter was transferred into zebrafish (Brachydanio rerio) by microinjection of fertilized eggs. Light emission could be monitored in vivo in eggs and throughout the early development of the fish by low-light video-image analysis. Gene expression was transient in most of the cases lasting for about 2 weeks. This gene cassette proved to be a very convenient and nondestructive transformation marker and the firefly luciferase gene appears to be a powerful tool for real-time imaging of tissue-specific gene expression in transgenic fish.

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