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Expression of firefly luciferase gene in Erwinia amylovora

✍ Scribed by Giovanna Gentilomi; Lisa Zeri; Severino Ghini; Marialuisa Zerbini; Elisa Zuffi; Monica Musiani; Stefano Girotti

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
359 KB
Volume
18
Category
Article
ISSN
1522-7235

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✦ Synopsis

Abstract

In this study we describe an efficient stable genetic transformation of the phytopathogenic bacterium Erwinia amylovora using a recombinant expression vector encoding the firefly luciferase gene of Photinus pyralis, which is further controlled by IPTG‐inducible promoter. Stably transformed E. amylovora cells maintain the same infectivity as the wild‐type strain and, after induction with IPTG, produce luciferase. Luminescence produced by the action of luciferase on an exogenous substrate was easily detectable by a simple and rapid bioluminescent assay (BL). The transformed E. amylovora strain maintains the same high emission level, even after passage in pears, until about 15 days post‐infection. Our findings therefore show that the luciferase assay can be conveniently used to follow the bacterial movement in plant tissue and its dissemination in controlled environments.

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