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Monitoring of reducing sugars by flow-injection analysis using p-hydroxybenzoic acid hydrazide

✍ Scribed by Peter Hartmann; Stephen J. Haswell; Manfred Grasserbauer

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
683 KB
Volume
285
Category
Article
ISSN
0003-2670

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✦ Synopsis

In this work, p-hydrorqbenxoic acid hydraxide has been used as a reagent to determine reducing sugars using flow-injection analysis with photometric detection (410 nm). Parameters such as the temperature, alkalinity, reactor length, injection volume, flows and reagent concentration were examined for their optimum conditions. Systems cata1yse.d by bismuth for low analyte concentrations and non-catalysed for higher levels, containing only a single sugar (sampling frequency 60-80 h-t) and a system with a longer residence time (sampling frequency 30 h-'1 in which the different responses of various sugars can be minimised, are described. Samples (fruits and beverages) measured with the latter system in the calibration range 0.08-0.6 g/l gave a typical R.S.D. of 2%.

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