Determination of depolymerization kinetics of amylose, amylopectin, and soluble starch by Aspergillus oryzae α-amylase using a fluorimetric 2-p-toluidinylnaphthalene-6-sulfonate/flow-injection analysis system

Determination of β-amylase activity by a

Determination of β-amylase activity by a fluorimetric 2-p-toluidinylnaphthalene-6-sulfonate flow-injection analysis (2,6-TNS–FIA) method, using amylose and amylopectin as substrates

✍ Natalia Batlle; José V. Carbonell; José M. Sendra 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 125 KB

2-p-Toluidinylnaphthalene-6-sulfonate (2,6-TNS) is a compound which is barely fluorescent in pure water but whose fluorescence can be strongly enhanced if the environment becomes hydrophobic, i.e. by the addition of suitable substrates such as proteins or 1,4-␣-Dglucans. The enhancement of fluoresce