Monitoring catalytic reaction of bilirubin oxidase and determination of bilirubin and bilirubin oxidase activity by capillary electrophoresis
β Scribed by Xiao-Mian Zhou; Jian-Wu Liu; Xiao Zou; Jhun-jin Chen
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 150 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
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Capillary electrophoresis (CE) is a promising technique for assessment of free bilirubin and its interaction with albumin, as it requires only a small sample volume and provides a rapid and efficient separation of free bilirubin from its albumin-bound complex in a one-phase system. In order to maint
## Abstract We investigated the enzymic activity of bilirubin oxidase when it is suspended as a lyophilized powder in a lowβwater system. The enzyme required buffer salts and a source of water to show activity. This study investigated the complete range of water thermodynamic activity (a~w~) by com