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Molecular Weight of Bacteriorhodopsin Solubilized in Triton X-100

โœ Scribed by Jacqueline A. Reynolds and Walther Stoeckenius

Book ID
123654552
Publisher
National Academy of Sciences
Year
1977
Tongue
English
Weight
506 KB
Volume
74
Category
Article
ISSN
0027-8424

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Molecular weight estimation of Triton X-
Molecular weight estimation of Triton X-100 solubilized proteins by polyacrylamide gel electrophoresis
โœ Vincent J. Hearing; Walter G. Klingler; Thomas M. Ekel; Paul M. Montague ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 632 KB

A polyacrylamide gel elec&ophoresis system is described which employs the nonionic detergent T&on X-100 as a protein solvent. Aside from the obvious advantages of this detergent over ionic detergents and urea in the preservation of protein structure and function, it is demonstrated that the ancillar