Spontaneous revertants of a temperature-sensitive Escherichia coli strain harboring a thermolabile leucyl-tRNA synthetase and seryl-tRNA synthetase were selected for growth at 40 degrees C. Among these, strains were found with increased levels of both thermolabile synthetases. Two distinct genetic l
Molecular weight distribution of the aminoacyl-tRNA-synthetases of Escherichia coli by gel filtration
✍ Scribed by Nass, Gisela ;Stöffler, Georg
- Publisher
- Springer
- Year
- 1967
- Tongue
- English
- Weight
- 263 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0026-8925
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A rapid gel filtration chromatography method is described for determination of the molecular weight distribution (MWD) of peptide mixtures by using calibrated Sephadex microbore columns. The method was applied to MWD analysis of peptide mixtures resulting from trypsin and pepsin digestion of glycini