Molecular species of extracellular phosphatidylethanolamine from Escherichia coli
β Scribed by L.M.G. Van Golde; L.L.M. Van Deenen
- Book ID
- 103038611
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 462 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0009-3084
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β¦ Synopsis
Phosphatidylethanolamine was hydrolysed by phospholipase C from Bacillus cereus (E.C. 3.1.4.3) and the resultant diglycerides were separated into five subfractions on thin-layer plates of silica impregnated with silver nitrate.
(2) The positional distribution of the fatty acids in these diglycerides was determined by means of hydrolysis with pancreatic lipase (E.C. 3.1.1.3). The results were in good agreement with those obtained by hydrolysis of the phosphatidylethanolamine with snake-venom phospholipase A (E.C. 3.1.1.4).
π SIMILAR VOLUMES
H--was grown in nutrient broth (Difco). Penieillinase activity was found in the culture supernatant after only five hours of incubation, i.e. during the exponential phase of growth. At this phase the levels of typical intracellular "markers", did not indicate cell lysis or gross cell damage. The bio