✍ Scribed by Tanco, Jos� M. ;Miravalles, Mar�a del Pilar ;Arcos, Jos� M.
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H--was grown in nutrient broth (Difco). Penieillinase activity was found in the culture supernatant after only five hours of incubation, i.e. during the exponential phase of growth. At this phase the levels of typical intracellular "markers", did not indicate cell lysis or gross cell damage. The bioautographic revelation of penicillin-splitting enzymes on electropherograms of cell-free liquids confirmed the presence of one basic broad-spectrum ~-laetamase. This possibly extraeellular/~-lactamase seems to be also present in the cellular extracts where it coexists with several other cell-bound penieillinases.
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Phosphatidylethanolamine was hydrolysed by phospholipase C from Bacillus cereus (E.C. 3.1.4.3) and the resultant diglycerides were separated into five subfractions on thin-layer plates of silica impregnated with silver nitrate. (2) The positional distribution of the fatty acids in these diglyceride