β Scribed by Indu Parikh, Pedro Cuatrecasas (auth.), That T. Ngo (eds.)
No coin nor oath required. For personal study only.
Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purificaΒ tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Front Matter....Pages i-xvii
Front Matter....Pages 1-1
Affinity Chromatography....Pages 3-13
Weak-Affinity Chromatography....Pages 15-25
Affinity Chromatography on Inorganic Support Materials....Pages 27-48
2-Fluoro-1-methylpyridinium (FMP) Salt-Activated Gels....Pages 49-68
Slalom Chromatography....Pages 69-87
Front Matter....Pages 89-89
Applications of Bacterial Immunoglobulin-Binding Proteins to the Purification of Immunoglobulins....Pages 91-112
Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins....Pages 113-126
Selective Isolation of C-Terminal Peptides by Affinity Chromatography....Pages 127-138
Receptor-Affinity Chromatography (RAC)....Pages 139-149
Immobilized Artificial Membrane Chromatography....Pages 151-167
Affinity Chromatography Using Immobilized Antisense-Family Peptides....Pages 169-177
Chromatographic Resolution of Chiral Compounds by Means of Immobilized Proteins....Pages 179-187
Chromatography with Cyclodextrin-Based Stationary Phases....Pages 189-202
Front Matter....Pages 203-203
Suitable Antibodies as Ligands in Affinity Chromatography of Biomolecules....Pages 205-211
Immunoaffinity Purification of Organelles....Pages 213-227
Immunoaffinity Separation of Cells Using Monosized Magnetic Polymer Beads....Pages 229-244
Front Matter....Pages 245-245
Affinity Chromatography with Immobilized Dyes....Pages 247-255
Pseudo-Biospecific Affinity Ligand Chromatography....Pages 257-275
Synthetic Protein Surface Domains as Bioactive Stationary Phases....Pages 277-312
Affinity Chromatography with Immobilized Benzeneboronates....Pages 313-322
Front Matter....Pages 245-245
Affinity Chromatographic Removal of Pyrogens....Pages 323-332
Molecular Interactions in Hydrophobic Chromatography....Pages 333-359
Front Matter....Pages 361-361
Biorecognition in Molecularly Imprinted Polymers....Pages 363-381
Bioseparation and Catalysis in Molecularly Imprinted Polymers....Pages 383-394
Use of Heterobifunctional Ligands in Affinity Chromatographic Processes....Pages 395-401
Covalent Chromatography....Pages 403-413
Aza-Arenophilic Interaction....Pages 415-430
Front Matter....Pages 431-431
Model Systems Employing Affinity Chromatography for Extraction of Toxic Substances Directly from Whole Blood....Pages 433-450
Applications of Affinity Binding to the Development of Heparin Removing and Sensing Devices....Pages 451-468
Affinity Precipitation....Pages 469-477
Affinity Separation of Nucleic Acids on Monosized Magnetic Beads....Pages 479-485
Affinity Ultrafiltration for Protein Purification....Pages 487-498
Affinity Partitioning of Biomolecules in Aqueous Two-Phase Systems....Pages 499-509
Affinity Electrophoresis of Macromolecules....Pages 511-531
Reversed Micelles for Protein Purification....Pages 533-544
Back Matter....Pages 545-570
Biochemistry, general; Biotechnology; Analytical Chemistry
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