Molecular interactions between adriamycin and x-ray damage in mammalian tumor cells

✍ Scribed by John E. Byfield; Young C. Lee; Lorna Tu

• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: French
• Weight: 771 KB
• Volume: 19
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910190208

✦ Synopsis

Abstract

The effect of the anthracycline antibiotic, Adriamycin (Ad), on the sedimentation properties of pre‐labelled mammalian DNA has been studied. Ad induces DNA degradation in vivo in both excision repair‐competent (HeLa and Me‐180) cells and in excision repair‐deficient (REQ) cells. When X‐irradiated cells are exposed to Ad during the period of repair of DNA single‐strand breaks, small numbers of residual breaks persist following completion of repair. These are attributable to those induced by Ad alone. The effects of Ad and X‐rays therefore appear to be similar and additive. No clear‐cut evidence that Ad can inhibit the repair of X‐ray‐induced DNA single‐strand breakage was found. Ad also induces the formation of DNA double‐strand breaks and inhibits the repair of X‐ray‐induced base damage (repair replication). The induction of DNA strand breakage may be responsible for Ad cell toxicity and may contribute to its capacity to enhance primary X‐irradiation damage when the two types of lesions co‐exist.