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Molecular identification of five commercial flatfish species by PCR–RFLP analysis of a 12S rRNA gene fragment

✍ Scribed by Angel S Comesaña; Paz Abella; Andrés Sanjuan

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
157 KB
Volume
83
Category
Article
ISSN
0022-5142

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✍ C�spedes, Ana; Garc�a, Teresa; Carrera, Esther; Gonz�lez, Isabel; Fern�ndez, Ali 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 147 KB

PCR±RFLP analysis was applied to the identi®cation of two closely related ¯at®sh species: sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides). Ampli®cation of DNA isolated from ®sh muscle samples was carried out using a set of primers ¯anking a region of 321 base pairs (bp) from