Genetic differentiation between sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides) by PCR-RFLP analysis of a 12S rRNA gene fragment
✍ Scribed by C�spedes, Ana; Garc�a, Teresa; Carrera, Esther; Gonz�lez, Isabel; Fern�ndez, Alicia; Asensio, Luis; Hern�ndez, Pablo E; Mart�n, Rosario
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 147 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
PCR±RFLP analysis was applied to the identi®cation of two closely related ¯at®sh species: sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides). Ampli®cation of DNA isolated from ®sh muscle samples was carried out using a set of primers ¯anking a region of 321 base pairs (bp) from the mitochondrial 12S rRNA gene. Restriction endonuclease analysis based on sequence data of this DNA fragment revealed the presence of polymorphic sites for AciI and MwoI endonucleases. The restriction pro®les obtained by agarose gel electrophoresis when amplicons were cut with AciI and MwoI enzymes allowed the unequivocal identi®cation of sole and Greenland halibut species.