𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Molecular identification and functional characterization of the vitamin C transporters expressed by Sertoli cells

✍ Scribed by Constanza Angulo; Maite A. Castro; Coralia I. Rivas; Dominique Segretain; Rodrigo Maldonado; Alejandro J. Yañez; Juan Carlos Slebe; Juan Carlos Vera; Ilona I. Concha

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
367 KB
Volume
217
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Vitamin C is an essential micronutrient for the development of male germ cells. In the gonad, the germ cells are isolated from the systemic circulation by the blood–testis barrier, which consists of a basal layer of Sertoli cells that communicate through an extensive array of tight junction complexes. To study the behavior of Sertoli cells as a first approach to the molecular and functional characterization of the vitamin C transporters in this barrier, we used the 42GPA9 cell line immortalized from mouse Sertoli cells. To date, there is no available information on the mechanism of vitamin C transport across the blood–testis barrier. This work describe the molecular identity of the transporters involved in vitamin C transport in these cells, which we hope will improve our understanding of how germ cells obtain vitamin C, transported from the plasma into the adluminal compartment of the seminiferous tubules. RT‐PCR analyses revealed that 42GPA9 cells express both vitamin C transport systems, a finding that was confirmed by immunocytochemical and immunoblotting analysis. The kinetic assays using radioactive vitamin C revealed that both ascorbic acid (AA) transporters, SVCT1 and SVCT2, are functionally active. Moreover, the kinetic characteristics of dehydroascorbic acid (DHA) and 3‐methylglucose (OMG) transport by 42GPA9 Sertoli cells correspond to facilitative hexose transporters GLUT1, GLUT2 and GLUT3 expressed in these cells. This data is consistent with the concept that Sertoli cells have the ability to take up vitamin C. It is an important finding and contributes to our knowledge of the physiology of male germ cells. J. Cell. Physiol. 217: 708–716, 2008. © 2008 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Functional characterization of protein v
Functional characterization of protein variants of the human multidrug transporter ABCC2 by a novel targeted expression system in fibrosarcoma cells
✍ Rudolf Arlanov; Andrew Porter; Dennis Strand; Rachel Brough; Darja Karpova; Rein 📂 Article 📅 2012 🏛 John Wiley and Sons 🌐 English ⚖ 596 KB

The multidrug resistance-associated protein 2 (MRP2/ABCC2) is involved in the efflux of endogenous and xenobiotic substrates, including several anticancer and antiviral drugs. The functional consequences of ABCC2 protein variants remain inconsistent, which may be due to shortcomings of the in vitro

Dose of 3-methylcholanthrene enhances vi
Dose of 3-methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells
✍ Yasuko Sone; Etsuko Ueta; Yasuko Sannomaru; Noriko Miyake; Hirohito Sone; Yuzuru 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 222 KB

## Abstract Polycyclic aromatic hydrocarbon (PAH) compounds including 3‐methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3‐methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats

Molecular and genomic characterization o
Molecular and genomic characterization of human DLEC, a novel member of the C-type lectin receptor gene family preferentially expressed on monocyte-derived dendritic cells
✍ Ignacio Arce; Pedro Roda-Navarro; María C. Montoya; Patricia Hernanz-Falcón; Ama 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 204 KB 👁 3 views

We have identified a novel gene encoding a protein designated DLEC (dendritic cell lectin), which is a type II membrane glycoprotein of 213 amino acids and belongs to the human calcium-dependent (C-type) lectin family. The cytoplasmic tail of DLEC lacks consensus signaling motifs and its extracellul

Characterization of the expression of va
Characterization of the expression of variant and standard CD44 in prostate cancer cells: Identification of the possible molecular mechanism of CD44/MMP9 complex formation on the cell surface
✍ B. Desai; T. Ma; J. Zhu; M.A. Chellaiah 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 593 KB

## Abstract CD44 is a glycosylated adhesion molecule and osteopontin is one of its ligand. CD44 undergoes alternative splicing to produce variant isoforms. Our recent studies have shown an increase in the surface expression of CD44 isoforms (sCD44 and v4–v10 variant CD44) in prostate cancer cells o