Dose of 3-methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells
✍ Scribed by Yasuko Sone; Etsuko Ueta; Yasuko Sannomaru; Noriko Miyake; Hirohito Sone; Yuzuru Otsuka; Kazuo Kondo; Tadao Kurata; Emiko Suzuki
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 222 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1095-6670
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✦ Synopsis
Abstract
Polycyclic aromatic hydrocarbon (PAH) compounds including 3‐methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3‐methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats were given l‐ascorbic acid daily and intraperitoneal injections of 10 mg 3‐methylcholanthrene in total. On day 10, vitamin C concentrations and the expression of vitamin C transporter in the tissues were measured. As a result, the levels of sodium‐dependent vitamin C transporter (SVCTs) 1 and the l‐ascorbic acid concentration in 3‐methylcholanthrene‐treated livers and hepatocytes have increased significantly. However, the content of vitamin C in the urine and TBARS in the liver have not changed. These results suggest that the administration of 3‐methylcholanthrene elevates the requirement for vitamin C via (SVCTs) 1 due to xenobitics‐metabolizing, such as the induction of cytochrome P450 family. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:369–376 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20403