Molecular Diagnostics in Cytopathology: A Practical Handbook for the Practicing Pathologist

✍ Scribed by Sinchita Roy-Chowdhuri, Paul A. VanderLaan, John M. Stewart, Gilda da Cunha Santos

Publisher: Springer International Publishing
Year: 2019
Tongue: English
Leaves: 531
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Molecular diagnostics are increasingly used to help guide targeted therapy in solid organ tumors and hematologic malignancies. A large proportion of molecular testing is performed on limited-volume samples obtained via minimally invasive techniques, such as fine needle aspiration. Increasingly, cytopathologists play an essential role in this process, both in the triage of specimens during rapid on-site evaluation and in the evaluation of archival samples to determine suitability for ancillary testing. Therefore, it is imperative that practicing cytopathologists stay abreast of up-to-date diagnostic, prognostic, and predictive ancillary tests that can be used on limited cytologic material. This is a challenge since the landscape of known genomic alterations is constantly evolving and the subsequent set of testing options is ever expanding.
The proposed text will provide a user-friendly quick-reference handbook to serve as a useful resource for practicing pathologists and laboratory personnel dealing with, and interested in, this evolving field of molecular cytopathology. Essential components to be presented include: 1) pre-analytic factors that affect sample selection and evaluation; 2) specimen preparation to maximize confidence in results; 3) interpretation of results; 4) potential limitations; and 5) workflow algorithms. In addition, specific disease specific molecular testing details will be outlined to provide the reader with resources for quick reference. All chapters will be written by experts in their fields and will include the most up-to-date scientific and clinical information.
Molecular Diagnostics in Cytopathology will be of value to Cytopathologists, Cytotechnologists, Cytotechnology students, Cytopathology fellows, Surgical pathologists, Pathology residents and fellows, Molecular Pathologists, Molecular pathology fellows, Molecular technologists, as well as Translational researchers with an interest in molecular cytopathology.

✦ Table of Contents

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
Introduction: Overview of Current Molecular Diagnostic Testing on Cytology Samples (Michael H. Roh, Rashmi Kanagal-Shamanna)....Pages 3-19
Front Matter ....Pages 21-21
The Cytology Specimen and Preparations: Advantages and Limitations (Gilda da Cunha Santos, Mauro Ajaj Saieg)....Pages 23-38
Sample Acquisition and Test Requisition (Ross A. Miller, Ashwyna Sunassee)....Pages 39-60
Pre-analytic Workflow and Specimen Evaluation (John M. Stewart)....Pages 61-81
DNA-Based Sequencing Assays (Pasquale Pisapia, Miriam Cieri, Francesco Pepe, Umberto Malapelle, Giancarlo Troncone)....Pages 83-97
RNA-Based Assays (Umberto Malapelle, Pasquale Pisapia, Miriam Cieri, Francesco Pepe, Giancarlo Troncone)....Pages 99-119
FISH Testing of Cytology Specimens: Pre-analytic, Analytic, and Post-analytic Considerations (Karen D. Tsuchiya, Laura J. Tafe, Julia A. Bridge)....Pages 121-159
Molecular Cytopathology Correlations: Interpretation of Molecular Diagnostic Results (Sinchita Roy-Chowdhuri)....Pages 161-178
Front Matter ....Pages 179-179
Human Papillomavirus (HPV) Testing of Head and Neck Cancers (Eleanor Russell-Goldman, Jeffrey F. Krane)....Pages 181-197
Human Papillomavirus (HPV) Testing on Cervical Cytology Specimens (Ming Guo)....Pages 199-221
Molecular Diagnostics in Lung Cytology (Paul A. VanderLaan)....Pages 223-247
Molecular Diagnostics in Thyroid Cytology (Michiya Nishino)....Pages 249-299
Molecular Diagnostics in Breast Cytology (Liza M. Quintana)....Pages 301-336
Molecular Diagnostics in Salivary Gland Cytology (Esther Diana Rossi, Zubair W. Baloch)....Pages 337-353
Molecular Diagnostics in Pancreatic and Biliary Cytology (Mingjuan Lisa Zhang, Martha Bishop Pitman)....Pages 355-376
Fluorescence In Situ Hybridization (FISH) Testing in Urinary Tract Cytology (Güliz A. Barkan, Stefan E. Pambuccian)....Pages 377-403
Molecular Diagnostics in Hematologic Malignancies (Rashmi Kanagal-Shamanna)....Pages 405-424
Molecular Diagnostics in Bone and Soft Tissue Tumors (Vickie Y. Jo, Xiaohua Qian)....Pages 425-489
Molecular Diagnostics in Pediatric Cytopathology (Maren Y. Fuller, Sara E. Monaco)....Pages 491-515
Molecular Cytopathology: Final Thoughts and Future Directions (Sinchita Roy-Chowdhuri)....Pages 517-528
Back Matter ....Pages 529-534

✦ Subjects

Medicine & Public Health; Pathology

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